FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate6_84.3510000005f7a5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate6_84.3510000005f7a5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60668
Sequences flagged as poor quality0
Sequence length27
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGAACGACAGCTGTCTCTTATACACA2720.448341794685831No Hit
CAGAACGACAGTGGCTGTCTCTTATAC620.10219555614162326No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAC53400.021.1953
ATAACGA950.021.1952
AGAACGA52850.021.1952
CTGAACG609.0039975E-1021.1951
GACCGAC2800.021.1953
CAGACCG2800.021.1951
CATAACG950.021.1951
CAGCACG2350.021.1949981
GCACGAC2350.021.1949983
AGCACGA2350.021.1949982
CAGAACG52850.021.1749481
ACAGTCT2850.020.967858
ACAGTCG5050.020.967858
ACAGTAG3500.020.967858
CAGTTTA456.2743675E-720.967859
AGCTTTT456.2743675E-720.9678518
CAGTCCG900.020.967859
CAGTCCC751.8189894E-1220.967859
CAGTTCT405.3429103E-620.967859
CAGTTCA405.3429103E-620.967859
Produced by FastQC (version 0.11.2)