FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate6_83.3510000005f78b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate6_83.3510000005f78b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25595
Sequences flagged as poor quality0
Sequence length27
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACAGAAGGACCTGTCTCTTATACACA2601.0158234030084001No Hit
CACAGAAGGACTGCTGTCTCTTATACA350.1367454580972846No Hit
CACAGAAGGACTGGGGGCTGTCTCTTA320.1250244188318031No Hit
CACAGAAGGACTCCTGTCTCTTATACA280.1093963664778277No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACCGAA1600.021.181821
CTCAGAA250.003129994321.1818181
CATAGAA303.574653E-421.1818181
CACAGAA22750.021.1818161
TAGAAGG303.6229298E-421.140043
ACAGAAG22850.021.140042
ACCGAAG1600.021.140042
TCAGAAG250.00316621821.140042
ATAGAAG303.6229298E-421.140042
CCGAAGG1600.021.140043
CAGAAGG23100.021.1400383
ACGTTTT354.421867E-520.97456218
ACTGAGA354.421867E-520.97456210
GGACTCA1100.020.9745628
GTTCTGT558.107236E-920.97456220
GAGTTTT354.421867E-520.97456218
CCTTTTT701.2732926E-1120.97456219
GGCTTTT354.421867E-520.97456218
GAATTTT354.421867E-520.97456218
GATTTTT1250.020.97456219
Produced by FastQC (version 0.11.2)