FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate6_81.3510000005f748.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate6_81.3510000005f748.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48362
Sequences flagged as poor quality0
Sequence length27
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGGAATGGCCTGTCTCTTATACACA1090.22538356560936273No Hit
AACGGAATGGCTGCTGTCTCTTATACA590.12199660890782019No Hit
AACGGAATGGCTGTCTCTTATACACAT550.1137256523716968No Hit
AACGGAATGGCTCCTGTCTCTTATACA530.10959017410363509No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGAAT250.0029718421.4190482
CGGAATG44850.021.4190483
CAGAATG250.0029718421.4190483
AACAGAA250.0029718421.4190481
AACGCAA455.063139E-721.4190461
AACGAAA701.0913936E-1121.4190461
ACGCAAT455.063139E-721.4190462
AGGAATG353.850308E-521.4190463
TCGGAAT353.850308E-521.4190462
ATGGAAT607.767085E-1021.4190462
ATCGGAA353.850308E-521.4190461
AAAGGAA353.850308E-521.4190461
AATGGAA607.767085E-1021.4190461
CGAAATG701.0913936E-1121.4190463
TGGAATG607.767085E-1021.4190463
AACGGAA44400.021.4190461
ACGAAAT701.0913936E-1121.4190462
CGCAATG455.063139E-721.4190463
ACGGAAT44550.021.3950082
TTGTCTG250.003397318820.93174718
Produced by FastQC (version 0.11.2)