FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate6_80.3510000005f711.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate6_80.3510000005f711.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42505
Sequences flagged as poor quality0
Sequence length27
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATAGTTAGCCCTGTCTCTTATACAC1520.35760498764851195No Hit
GGATAGTTAGCCTGTCTCTTATACACA810.19056581578637807No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATCGT2650.021.4782621
GGATAGT35350.021.4782621
ATAGTTA35500.021.4782623
ATCGTTA2650.021.4782623
GATAGTT35400.021.4782622
CTAGTTA1600.021.478263
GGCTAGT1600.021.478261
TTAGTTA1300.021.478263
GATCGTT2700.021.0805152
AGGGGTT250.003399459620.92235416
AGCCCGA303.9458324E-420.9223549
TGGTCTG250.003399459620.92235418
AGCCCCC303.9458324E-420.9223549
AGCCTCT601.033186E-920.9223549
AGCCTCC250.003399459620.9223549
AGCCTCA354.5996625E-520.9223549
AGCCCAC250.003399459620.9223549
AGCCCAA507.426024E-820.9223549
AACGTTT250.003399459620.92235417
TCCTTTT354.5996625E-520.92235418
Produced by FastQC (version 0.11.2)