FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate6_76.3510000005f6a6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate6_76.3510000005f6a6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35067
Sequences flagged as poor quality0
Sequence length27
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGGACGAGATCTGTCTCTTATACAC2510.715772663757949No Hit
AGAGGACGAGCTGTCTCTTATACACAT1360.3878290130321955No Hit
AGAGGACGAGATCTGTCTCTTCTACAC820.23383808138705905No Hit
AGAGGACGAGATGCTGTCTCTTATACA500.14258419596771896No Hit
AGAGGACGAGCTGTCTCTTATCCACAT440.12547409245159266No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGGAC31000.021.4743791
GAGAACG250.00291121721.4743772
GAGGACG30950.021.4743772
AGGACGA31200.021.4743773
AGTGGAC454.8562106E-721.4743771
CGGACGA1950.021.4743773
GCGGACG1950.021.4743772
AGAACGA250.00291121721.4743773
AGCGGAC1950.021.4743771
AGAGAAC250.00291121721.4743771
GATGGGT601.0131771E-920.92296810
GATGGGA303.925958E-420.92296810
TTGTTTT900.020.92296818
GATGGCG354.568984E-520.92296810
GATGGCC303.925958E-420.92296810
ATGGGCG303.925958E-420.92296811
GCCTTTT601.0131771E-920.92296818
GGCATTT354.568984E-520.92296817
ATCTTTT405.3352414E-620.92296818
GGGCATT303.925958E-420.92296816
Produced by FastQC (version 0.11.2)