FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate6_75.3510000005f68c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate6_75.3510000005f68c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15528
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCGACCAACGACTGTCTCTTATACAC210.13523956723338484No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACC13700.021.4768221
CGACCAA13950.021.4768223
TCGACCA13650.021.476822
TTCGCCC657.094059E-1121.476821
CGCCCAA657.094059E-1121.476823
TCGCCCA657.094059E-1121.476822
AACGAGT1400.020.9225838
AACGAGC1250.020.9225838
AACGACT354.360763E-520.9225838
TTTCTGT354.360763E-520.92258321
ACGAGGT354.360763E-520.9225839
TTCTTTT354.360763E-520.92258318
ACGAGCA354.360763E-520.9225839
GCGTTTT354.360763E-520.92258318
ATGTTTT354.360763E-520.92258318
AAGTTTT354.360763E-520.92258318
TGGTTTT405.0243852E-620.9225818
TCCTTTT303.7911048E-420.9225818
AGGGGGG250.003303651520.9225812
GTGTTTT506.686241E-820.9225818
Produced by FastQC (version 0.11.2)