FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate6_73.3510000005f649.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate6_73.3510000005f649.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11251
Sequences flagged as poor quality0
Sequence length27
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGGTTCGTTGCTGTCTCTTATACAC310.27553106390543064No Hit
CTTGGTTCGTTGCTGTCTCTTCTACAC210.1866500755488401No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTTCG10050.021.2432443
GGGTTCG404.2173124E-621.2432423
CTGGGTT404.2173124E-621.2432421
CTTGGTT9800.021.2432421
TTGGTTC9800.021.2432422
TGGGTTC404.2173124E-621.2432422
GTGTTTT303.657035E-420.96000118
GTTGAGC303.657035E-420.9600019
GTTGACT303.657035E-420.9600019
GTTGAAG303.657035E-420.9600019
GTTGAAC303.657035E-420.9600019
TTTTTTT8950.020.96000121
GGATTTT404.747677E-620.96000118
GGTTTTT1300.020.96000119
CATTTTT404.747677E-620.96000119
TTGGGGG303.657035E-420.96000110
GTCTTTT303.657035E-420.96000118
GATTTTT1250.020.96000119
GTTGGGT303.657035E-420.9600019
GTTGGCG303.657035E-420.9600019
Produced by FastQC (version 0.11.2)