FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate6_71.3510000005f5f7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate6_71.3510000005f5f7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21643
Sequences flagged as poor quality0
Sequence length27
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGCTATCCCTGTCTCTTATACACA1330.6145173959247794No Hit
ATCTGCTATCCTGTCTCTTATACACAT420.1940581250288777No Hit
ATCTGCTATCCGGCTGTCTCTTATACA240.11089035715935867No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCTA250.002912165121.426542
ATCTACT454.777212E-721.426541
TCTACTA454.777212E-721.426542
TCTGCTA19300.021.426542
GCTGCTA454.777212E-721.426542
ATGCTAT250.002912165121.426543
CTGCTAT19950.021.426543
ATCTGCT19350.021.426541
AGCTGCT454.777212E-721.426541
TTTGCTA250.002912165121.426542
CTACTAT454.777212E-721.426543
TTGCTAT250.002912165121.426543
GGGTTTT950.020.93055718
CAGTTTT455.9986814E-720.93055718
GGCGTTT405.1632232E-620.93055717
TGTTTTT1850.020.93055719
ACTTTTT800.020.93055719
TCCGGTA455.9986814E-720.9305579
TCCGGGT455.9986814E-720.9305579
TCCGGGG1800.020.9305579
Produced by FastQC (version 0.11.2)