FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate6_7.3510000005ee00.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate6_7.3510000005ee00.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46912
Sequences flagged as poor quality0
Sequence length27
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCAGCAATGCCTGTCTCTTATACAC1300.2771145975443383No Hit
CTTCAGCAATGCGGGGGGGGTTTTTTT700.14921555252387447No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACAGC506.3222615E-821.2529741
ACAGCAA506.3222615E-821.2529743
CTTCAGC38800.021.2529741
CGTCAGC608.54925E-1021.2529741
TACAGCA506.3222615E-821.2529742
CCAGCAA608.54925E-1021.2529743
TAAGCAA250.00310812421.2529743
CTCCAGC608.54925E-1021.2529741
TTCTGCA404.7126196E-621.2529742
TCCAGCA608.54925E-1021.2529742
TCTGCAA404.7126196E-621.2529743
TTAAGCA250.00310812421.2529742
CTTAAGC250.00310812421.2529741
TTCAGCA38750.021.2529742
GTCAGCA608.54925E-1021.2529742
CTTCTGC404.7126196E-621.2529741
TTCCGCA2950.021.2529722
TCAGCAA39750.021.2529723
GCAGCAA1400.021.2529723
TCCGCAA2950.021.2529723
Produced by FastQC (version 0.11.2)