FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate6_69.3510000005f5c0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate6_69.3510000005f5c0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71852
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTTGAACTGGGGCTGTCTCTTATAC1030.1433502198964538No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTTGA1600.021.4639551
ACCTCGA250.002950010621.4639551
ACCTTGA66050.021.4639551
CCTTGAA66100.021.4486472
CTCGAAC250.002962269121.4486453
TTTGAAC1600.021.4486453
CATTGAA701.0913936E-1121.4486452
CCTCGAA250.002962269121.4486452
ATTGAAC701.0913936E-1121.4486453
CTAGAAC404.406298E-621.4486453
CTTGAAC67100.021.4486453
CTTTGAA1600.021.4486452
CCTAGAA404.406298E-621.4486452
CTGGGGA2150.020.9262379
CTGGCGA850.020.9262379
CTGGCCT850.020.9262379
CTGGTAT850.020.9262379
ACTGGTT3400.020.9262378
ACTGGCG5550.020.9262378
ACTGGCA3650.020.9262378
Produced by FastQC (version 0.11.2)