FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate6_66.3510000005f563.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate6_66.3510000005f563.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27371
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATTGGATACCGGCTGTCTCTTATAC990.3616966862737934No Hit
GCATTGGATACCCTGTCTCTTATACAC960.35073618062913303No Hit
GCATTGGATACCTGTCTCTTATACACA820.29958715428738447No Hit
GCATTGGATACCAGCTGTCTCTTATAC500.18267509407767346No Hit
GCATTGGATACCGCCTGTCTCTTATAC440.16075408278835265No Hit
GCATTGGATACCTGCTGTCTCTTATAC440.16075408278835265No Hit
GCATTGGATACCCGCTGTCTCTTATAC430.15710058090679918No Hit
GCATTGGATACCGTCTGTCTCTTATAC410.14979357714369224No Hit
GCATTGGATACCACCTGTCTCTTATAC410.14979357714369224No Hit
GCATTGGATACCCTGTCTCTTCTACAC390.1424865733805853No Hit
GCATTGGATACCATCTGTCTCTTATAC360.1315260677359249No Hit
GCATTGGATACCCCTGTCTCTTATACA360.1315260677359249No Hit
GCATTGGATACCCCCTGTCTCTTATAC310.11325855832815754No Hit
GCATTGGATACTGTCTCTTATACACAT290.10595155456505059No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTGGAT24200.021.403743
CTTGGAT1650.021.403743
CATTGGA23650.021.4037382
GTATTGG454.928643E-721.4037381
TATTGGA454.928643E-721.4037382
TTTGGAT404.3176206E-621.4037383
CTTTGGA404.3176206E-621.4037382
GCATTGG23650.021.4037381
GCTTTGG404.3176206E-621.4037381
TAGTTTT558.354618E-920.93418918
TACCGGC1100.020.9341899
TACCGCT558.354618E-920.9341899
TACCGCC558.354618E-920.9341899
AGGTTTT558.354618E-920.93418918
TACCTCG558.354618E-920.9341899
TACCCAG558.354618E-920.9341899
CTGTTTT558.354618E-920.93418918
ATACCAG2200.020.9341898
TACCAAG558.354618E-920.9341899
TTGTTTT405.2399573E-620.93418718
Produced by FastQC (version 0.11.2)