FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate6_63.3510000005f506.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate6_63.3510000005f506.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31386
Sequences flagged as poor quality0
Sequence length27
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGCATAGCTGCTGTCTCTTATACACA1870.5958070477282865No Hit
TGGCATAGCTGAGGGGCTGTCTCTTAT800.25489071560568405No Hit
TGGCATAGCTGAAGGGCTGTCTCTTAT530.1688650990887657No Hit
TGGCATAGCTGAGGGGGGGGTTTTTTT530.1688650990887657No Hit
TGGCATAGCTGAGGGGGCTGTCTCTTA490.15612056330848148No Hit
TGGCATAGCTGAGGCTGTCTCTTATAC470.1497482954183394No Hit
TGGCATAGCTGACGGGCTGTCTCTTAT350.11151468807748678No Hit
TGGCATAGCTGAGAGGCTGTCTCTTAT320.10195628624227362No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGCATA505.683978E-821.4110951
TGCATAG505.683978E-821.4110952
GCCTAGC2000.021.4110953
GGCCTAG2000.021.4110952
TGGCCTA2000.021.4110951
TGGCATA26800.021.4110931
GTCATAG709.094947E-1221.4110932
GGCATAG26800.021.4110932
TGTCATA709.094947E-1221.4110931
TGGAATA303.3462365E-421.4110931
GAATAGC303.3462365E-421.4110933
GGAATAG303.3462365E-421.4110932
TCATAGC709.094947E-1221.4110933
GCATAGC27300.021.4110933
TGAGAAA250.003369814520.93301610
TGGTTTT507.230665E-820.93301618
GAGCGGC250.003369814520.93301611
CTGAGTG1050.020.9330169
CTGAGGT1000.020.9330169
CTGAGGG3450.020.9330169
Produced by FastQC (version 0.11.2)