FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate6_59.3510000005f48e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate6_59.3510000005f48e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45336
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGTGCCTCAGCTGTCTCTTATACACA690.1521969295923769No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTACC850.021.5551781
AAGTCCC250.002861413421.5551781
ATGTGCC709.094947E-1221.5551761
AAGTGCC41400.021.5551761
TTGCCTC607.239578E-1021.5306843
GTACCTC850.021.5306823
TGTGCCT709.094947E-1221.5306822
GTGCCTC42100.021.5306823
AGTGCCT41550.021.4788632
TCCTTTT250.003411905120.91280618
GCCTTTT507.489507E-820.91280618
CAGTCTA558.8366505E-920.9128069
GTTTGGG250.003411905120.91280611
GGCATTT250.003411905120.91280617
GTGTCGG250.003411905120.91280611
CAGTCGC507.489507E-820.9128069
CAGTCGA558.8366505E-920.9128069
CAGTCCC558.8366505E-920.9128069
CAGTCCA303.9635974E-420.9128069
CAGTTCT601.0440999E-920.9128069
Produced by FastQC (version 0.11.2)