FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate6_51.3510000005f38f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate6_51.3510000005f38f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34807
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGAACAGAGTCTGTCTCTTATACAC3561.022782773580027No Hit
GAGGAACAGAGTCTGTCTCTTCTACAC1280.36774212083776253No Hit
GAGGAACAGAGCTGTCTCTTATACACA1210.34763122360444737No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCACAG2400.021.3964773
AGGAACA30400.021.3964772
AGGCACA2400.021.3964772
GGAACAG30900.021.3964773
GGTACAG303.3723906E-421.3964773
GAGGAAC30250.021.3964751
ATGAACA250.002973083221.3964752
GATGAAC250.002973083221.3964751
TGGAACA505.7731086E-821.3964752
TGAACAG250.002973083221.3964753
GTGGAAC505.7731086E-821.3964751
GAGGCAC2450.020.9598121
GCCTTTT405.3061685E-620.93534718
GAGTGGC1550.020.9353479
GAGTGCA405.3061685E-620.9353479
TTTCTGT405.3061685E-620.93534721
CGCTTTT405.3061685E-620.93534718
AGTGGTG405.3061685E-620.93534710
AGTGGCG800.020.93534710
AGTGGCC405.3061685E-620.93534710
Produced by FastQC (version 0.11.2)