FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate6_50.3510000005f365.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate6_50.3510000005f365.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16910
Sequences flagged as poor quality0
Sequence length27
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTAGTGAGCTAGGGCTGTCTCTTATA340.2010644589000591No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGTGA455.1186E-721.215572
GGCAGTG455.1186E-721.215571
CAGTGAG455.1186E-721.215573
GAGTGAG353.9410523E-521.2155693
GTCGTGA950.021.2155692
TCGTGAG950.021.2155693
GGAGTGA353.9410523E-521.2155692
GGTCGTG950.021.2155691
GTAGTGA14600.021.2155672
TAGTGAG14700.021.1434063
GGTAGTG14650.021.143161
GCTAGCA455.748552E-720.9644999
GCTAGAG850.020.9644999
AGCTAGT1750.020.9644998
GGATTTT455.748552E-720.96449918
TATTTTT900.020.96449919
GACTTTT455.748552E-720.96449918
ATCTGTC455.748552E-720.96449920
AGGGGTG250.003277738820.96449712
TGGTTTT303.7598007E-420.96449718
Produced by FastQC (version 0.11.2)