FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate6_5.3510000005edcb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate6_5.3510000005edcb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42574
Sequences flagged as poor quality0
Sequence length27
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCTACGTCTGTCTCTTATACACATC1300.30535068351576083No Hit
GAGCTACGTCTAGGGGCTGTCTCTTAT530.12448912481796402No Hit
GAGCTACGTCTCTGTCTCTTATACACA520.12214027340630432No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTACG607.730705E-1021.4110582
GATCTAC607.730705E-1021.4110581
TGCTACG950.021.4110582
GTGCTAC950.021.4110581
GATACGT404.4017233E-621.4110573
AGCTACG39000.021.4110572
GAGCTAC38950.021.4110571
GCTACGT39900.021.4110573
AGATACG404.4017233E-621.4110572
TGGTTTT900.020.93302218
GCCTTTT601.02591E-920.93302218
CAGTTTT601.02591E-920.93302218
ATCTTTT601.02591E-920.93302218
AGCTTTT751.8189894E-1220.93302218
TCTAGTG1200.020.9330229
TCTAGTA751.8189894E-1220.9330229
CTATAGT303.932382E-420.93302210
CTATAGG303.932382E-420.93302210
TAGTGGG303.932382E-420.93302211
TCTAGGT1200.020.9330229
Produced by FastQC (version 0.11.2)