FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate6_47.3510000005f2fa.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate6_47.3510000005f2fa.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25592
Sequences flagged as poor quality0
Sequence length27
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCAGACTGGAGGCTGTCTCTTATAC550.21491090965926854No Hit
TAGCAGACTGGACCTGTCTCTTATACA430.16802125664270084No Hit
TAGCAGACTGGAGCTGTCTCTTATACA400.15629884338855893No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGACT22100.021.3960023
TTGCAGA250.00295154721.3961
TAGCCGA1700.021.3961
TAGCAGA21850.021.3961
ATCAGAC658.367351E-1121.3962
AGCAGAC21850.021.3962
TCAGACT658.367351E-1121.3963
TATCAGA658.367351E-1121.3961
TGCAGAC250.00295154721.3962
GCCGACT1700.021.3963
TAGCGGA303.3381558E-421.3959981
AGCGGAC303.3381558E-421.3959982
TTGTTTT354.4859946E-520.93542118
GGATGTG354.4859946E-520.93542110
GGAGAGG250.00335066920.93542110
GGAGACT250.00335066920.93542110
GGATGGG405.2148243E-620.93542110
GTGTTTT651.1277734E-1020.93542118
GGGTTTT850.020.93542118
GCCTTTT354.4859946E-520.93542118
Produced by FastQC (version 0.11.2)