FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate6_46.3510000005f2d0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate6_46.3510000005f2d0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44021
Sequences flagged as poor quality0
Sequence length27
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TACTGCAAGGACTGTCTCTTATACACA1420.3225733172803889No Hit
TACTGCAAGGAGGCTGTCTCTTATACA630.1431135140046796No Hit
TACTGCAAGGAGGGGGGGCTGTCTCTT490.11131051089252858No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTGCA40800.021.4400481
CTGCAAG41150.021.4400483
CTACAAG556.6065695E-921.4400463
ACTGCAA40750.021.4400462
TTGCAAG505.738366E-821.4400463
TTCTGCA404.355872E-621.4400461
CTCCAAG404.355872E-621.4400463
ACTCCAA404.355872E-621.4400462
TATTGCA505.738366E-821.4400461
TCTGCAA404.355872E-621.4400462
ATTGCAA505.738366E-821.4400462
TACTCCA404.355872E-621.4400461
TACTACA556.6065695E-921.4400461
ACTACAA556.6065695E-921.4400462
AAGGAGG20350.020.9284117
GGAGATA456.306873E-720.928419
TGGTTTT1150.020.9284118
GAGGCGA405.3748226E-620.9284110
GAGGTGA456.306873E-720.9284110
GAGGCCA250.003395648420.9284110
Produced by FastQC (version 0.11.2)