FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate6_44.3510000005f29d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate6_44.3510000005f29d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27173
Sequences flagged as poor quality0
Sequence length27
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGACA800.021.269161
AGCCACT24600.021.269163
TAGCCAC24200.021.269162
AGACACT800.021.269163
TTAGCCA24200.021.269161
TAGACAC800.021.269162
TTCGCCA1000.021.2691571
TCGCCAC1000.021.2691572
CTCTGGT3050.020.9558018
CTCTGGG4200.020.9558018
CTCTGGC2700.020.9558018
CTCTGGA1200.020.9558018
TTGTTTT701.2732926E-1120.95580118
TGGTTTT609.640644E-1020.95580118
CTCTGCT1150.020.9558018
CTCTGCG1350.020.9558018
CTCTGCC1300.020.9558018
CTCTGCA1600.020.9558018
CTCTGAT1250.020.9558018
CTCTGAG1650.020.9558018
Produced by FastQC (version 0.11.2)