FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate6_43.3510000005f273.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate6_43.3510000005f273.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24343
Sequences flagged as poor quality0
Sequence length27
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAGCTGGATGCTGTCTCTTATACACA2050.8421312081501869No Hit
CTAGCTGGATCTGTCTCTTATACACAT500.20539785564638705No Hit
CTAGCTGGATGTCTGTCTCTTATACAC350.14377849895247094No Hit
CTAGCTGGATGTGGGGGCTGTCTCTTA260.10680688493612125No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTGGA1250.021.187113
TAGCTGG20700.021.187112
GAGCTGG354.0781735E-521.187112
TCGCTGG1250.021.187112
CTAGCTG20800.021.187111
CGAGCTG354.0781735E-521.187111
CTCGCTG1250.021.187111
AGCTGGA21250.021.187113
TGCTGGA751.8189894E-1221.1871093
AGATGGA506.209302E-821.1871093
CTAGATG506.209302E-821.1871091
TAGATGG506.209302E-821.1871092
TTGCTGG751.8189894E-1221.1871092
TGTTGGG609.422365E-1020.96913710
CAGTTTT405.1253755E-620.96913718
AGCTTTT609.422365E-1020.96913718
GATGTTG2150.020.9691378
GATGTTC850.020.9691378
GATGTTA800.020.9691378
GATGTCA850.020.9691378
Produced by FastQC (version 0.11.2)