FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate6_4.3510000005eda2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate6_4.3510000005eda2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8224
Sequences flagged as poor quality0
Sequence length27
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGACGTCGATACCTGTCTCTTATACAC1351.641536964980545No Hit
AGACGTCGATACCTGTCTCTTCTACAC320.38910505836575876No Hit
AGACGTCGATACGCTGTCTCTTATACA130.1580739299610895No Hit
AGACGTCGATACGGGGGGCTGTCTCTT100.12159533073929961No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCGA7250.021.453
CCGTCGA353.32251E-521.453
AGACGTC7300.021.451
GACGTCG7300.021.452
GCCGTCG353.32251E-521.452
AGCCGTC353.32251E-521.451
GTGTTTT250.003169200420.9268318
GGGTTTT303.5775185E-420.9268318
CGATACT1800.020.926837
CGATACC1700.020.926837
CGATACA1550.020.926837
GATACTT505.7440047E-820.926838
TATACAC250.003169200420.9268321
GATACTC303.5775185E-420.926838
AATTTTT303.5775185E-420.9268319
GATACGT709.094947E-1220.926838
GATACCT607.166818E-1020.926838
GATACCG505.7440047E-820.926838
GATACCC303.5775185E-420.926838
GATACCA303.5775185E-420.926838
Produced by FastQC (version 0.11.2)