FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate6_39.3510000005f209.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate6_39.3510000005f209.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25987
Sequences flagged as poor quality0
Sequence length27
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTCGTCAGCCTGTCTCTTATACACA350.1346827259783738No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGTCA21950.021.2460943
ACGTCGT659.276846E-1121.2460941
GTCGTCA659.276846E-1121.2460943
CCTCGTC354.004744E-521.2460942
CTTAGTC1900.021.2460942
ACATCGT404.59048E-621.2460941
ACTTCGT21350.021.2460941
ACTTAGT1900.021.2460941
ACCTCGT354.004744E-521.2460941
CTTCGTC21350.021.2460942
CGTCGTC659.276846E-1121.2460942
ATCGTCA404.59048E-621.2460943
ATTTCGT659.276846E-1121.2460941
TTAGTCA1900.021.2460943
TTTCGTC659.276846E-1121.2460942
CATCGTC404.59048E-621.2460942
TTGTTTT405.168271E-620.9595418
AGCTTGG800.020.959549
AGCTTAT405.168271E-620.959549
AGCTTAA405.168271E-620.959549
Produced by FastQC (version 0.11.2)