FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate6_38.3510000005f1ee.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate6_38.3510000005f1ee.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31846
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTGCCGAACCTGTCTCTTATACACA810.25434905482635184No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATGCC454.718513E-721.5242731
TTACCGA607.021299E-1021.5242733
GATGCCG454.718513E-721.5242732
TGCTGCC303.2299245E-421.5242731
TGTTACC607.021299E-1021.5242731
GCTGCCG303.2299245E-421.5242732
ATGCCGA454.718513E-721.5242733
CTGCCGA303.2299245E-421.5242733
GTTACCG607.021299E-1021.5242732
TTGCCGA28450.021.5242733
TGTTGCC28250.021.5242711
GTTGCCG28300.021.5242712
GTGCCGA800.021.5242713
AACAAGA456.231621E-720.9150969
GCGGTTT456.231621E-720.91509617
GGATTTT900.020.91509618
CAGGGGG651.1823431E-1020.91509611
TCGTTTT456.231621E-720.91509618
AGGTTTT900.020.91509618
GAACAAA900.020.9150968
Produced by FastQC (version 0.11.2)