FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate6_36.3510000005f1ab.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate6_36.3510000005f1ab.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18422
Sequences flagged as poor quality0
Sequence length27
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGAACGACAGCTGTCTCTTATACACA1180.6405384865921181No Hit
CAGAACGACAGTGGCTGTCTCTTATAC320.17370535229616763No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCACG751.8189894E-1221.1978021
TAACGAC303.5033573E-421.1978023
GAACGAC16450.021.1978023
ATAACGA303.5033573E-421.1978022
GCACGAC751.8189894E-1221.1978023
GACCGAC556.833943E-921.1978023
CAGACCG556.833943E-921.1978021
AGCACGA751.8189894E-1221.1978022
AGACCGA556.833943E-921.1978022
CAGAACG16350.021.1978021
CATAACG303.5033573E-421.1978021
AGAACGA16400.021.1331752
CGACAGT18150.020.9673926
ACAGTGT1450.020.9673928
ACAGTGC2250.020.9673928
ACAGTGA1650.020.9673928
TGGTTTT354.3467087E-520.96739218
ACAGTCT751.8189894E-1220.9673928
ACAGTCG1650.020.9673928
ACAGTCC701.2732926E-1120.9673928
Produced by FastQC (version 0.11.2)