FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate6_35.3510000005f181.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate6_35.3510000005f181.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35992
Sequences flagged as poor quality0
Sequence length27
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACAGAAGGACCTGTCTCTTATACACA3340.927983996443654No Hit
CACAGAAGGACTGGGGGCTGTCTCTTA520.144476550344521No Hit
CACAGAAGGACTGGGGCTGTCTCTTAT400.11113580795732383No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGAAGG557.612471E-921.1514743
ATAGAAG557.612471E-921.1514742
CATAGAA557.612471E-921.1514741
ACAAAAG250.003181410321.1514722
ACAGAAG32100.021.1514722
CACAAAA250.003181410321.1514721
CACCGAA2000.021.1514721
ACCGAAG2000.021.1514722
CAGAAGG32450.021.1514723
CAAAAGG250.003181410321.1514723
CCGAAGG2000.021.1514723
CACAGAA32100.021.1185261
ACTGCTC354.4865526E-520.97496610
ACTGTTC250.003340373520.97496610
TCCTTTT250.003340373520.97496618
ACTGCGT250.003340373520.97496610
ACTGCGG354.4865526E-520.97496610
ACTGTGT354.4865526E-520.97496610
ACTGTGG701.2732926E-1120.97496610
ACTGCCG354.4865526E-520.97496610
Produced by FastQC (version 0.11.2)