FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate6_34.3510000005f167.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate6_34.3510000005f167.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23029
Sequences flagged as poor quality0
Sequence length27
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTCGTCTCGATCTGTCTCTTATACAC1310.5688479742932824No Hit
ACTCGTCTCGATCTGTCTCTTCTACAC530.23014460028659517No Hit
ACTCGTCTCGATGCTGTCTCTTATACA320.13895523036171784No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCGTC20900.021.2781451
TTCGTCT303.451124E-421.2781452
ACCCGTC303.451124E-421.2781451
CCGTCTC303.451124E-421.2781453
CCCGTCT303.451124E-421.2781452
ATTCGTC303.451124E-421.2781451
TCGTCTC21150.021.2781453
GCGTCTC607.767085E-1021.2781453
ACGCGTC607.767085E-1021.2781451
CTCGTCT20900.021.227242
GATGGGG701.2732926E-1120.9543510
TGGTTTT558.089046E-920.9543518
ATGGGGG354.429825E-520.9543511
GTGTTTT651.09139364E-1020.9543518
AGCTTTT354.429825E-520.9543518
CGATACC354.429825E-520.954359
TCGATCG1400.020.954358
TCGATAA651.09139364E-1020.954358
ATAGGGG354.429825E-520.9543511
TGTTTTT2350.020.9543519
Produced by FastQC (version 0.11.2)