FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate6_32.3510000005f124.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate6_32.3510000005f124.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33292
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATAGTTAGCCCTGTCTCTTATACAC1610.4835996635828428No Hit
GGATAGTTAGCCTGTCTCTTATACACA610.18322720173014537No Hit
GGATAGTTAGCCCTGTCTCTTCTACAC560.16820857863751051No Hit
GGATAGTTAGCCGGGGGGGGTTTTTTT360.10813408626697105No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTAGTT1400.021.4722232
GATCGTT1750.021.4722232
GGATCGT1750.021.4722231
GGTTAGT1050.021.4722231
GTTAGTT1050.021.4722232
CTAGTTA1400.021.4722233
GGCTAGT1400.021.4722231
ATCGTTA1750.021.4722233
TTAGTTA1050.021.4722233
ATAGTTA28050.021.4722213
GGATAGT27900.021.4337431
GATAGTT27950.021.3953972
AGCCCGG701.4551915E-1120.923319
AGCCTGG507.299968E-820.923319
AGCCTGA354.559288E-520.923319
AGCCCCT354.559288E-520.923319
AGCCTCG558.572897E-920.923319
AGCCTCA250.00338316620.923319
AGCCTAT354.559288E-520.923319
AGCCTAG354.559288E-520.923319
Produced by FastQC (version 0.11.2)