FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate6_30.3510000005f0d2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate6_30.3510000005f0d2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21713
Sequences flagged as poor quality0
Sequence length27
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTGTGACGAACCTGTCTCTTATACAC760.35002072491134345No Hit
AGTGTGACGAACCTGTCTCTTCTACAC300.13816607562289873No Hit
AGTGTGACGAACGGGGGGGGTTTTTTT240.110532860498319No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTGAC250.002845774221.5118482
GCGTGAC555.9044396E-921.5118482
AGAGTGA250.002845774221.5118481
AGTGTGA19000.021.5118481
AGTGAGA303.1953564E-421.5118481
AGTGACG250.002845774221.5118483
AGCGTGA555.9044396E-921.5118481
CGTGACG555.9044396E-921.5118483
AGGGTGA751.8189894E-1221.5118471
TGTGACG19100.021.4555343
GTGTGAC19050.021.4553872
TGGTTTT900.020.91705118
AACGCGG354.4759043E-520.91705110
AACGTGG456.0387356E-720.91705110
GTGTTTT950.020.91705118
AACGTAG303.866883E-420.91705110
AGCTTTT354.4759043E-520.91705118
GTCGTTT303.866883E-420.91705117
GGCGTTT405.1940297E-620.91705117
AACGAGG303.866883E-420.91705110
Produced by FastQC (version 0.11.2)