FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate6_29.3510000005f0b8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate6_29.3510000005f0b8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39151
Sequences flagged as poor quality0
Sequence length27
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGTCCAAGCCTGTCTCTTATACACA2730.6973001966744145No Hit
AAGGTCCAAGCACTGTCTCTTATACAC2530.6462159331817834No Hit
AAGGTCCAAGCACTGTCTCTTCTACAC1050.26819238333631323No Hit
AAGGTCCAAGCAGTGGTATCAACGCAG580.14814436412863016No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTCCA303.3033398E-421.4718232
AGGACCA607.403287E-1021.4718232
GGACCAA607.403287E-1021.4718233
ATGGTCC303.3033398E-421.4718231
AAGGTCC36250.021.4718231
AGGTCCA36250.021.4718232
AAGGACC607.403287E-1021.4718231
TGTCCAA250.002919453221.471823
GGTCCAA36550.021.471823
GGCCCAA353.7532962E-521.471823
AGGCCCA353.7532962E-521.471822
AAGGCCC353.7532962E-521.471821
ATGTCCA250.002919453221.471822
AATGTCC250.002919453221.471821
TGTTGTT250.003393760420.92337216
TTGTTTT701.4551915E-1120.92337218
TGGTTTT900.020.92337218
TCCTTTT354.5859902E-520.92337218
GGGTCTG250.003393760420.92337218
GTGTTTT558.69295E-920.92337218
Produced by FastQC (version 0.11.2)