FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate6_26.3510000005f05c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate6_26.3510000005f05c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35267
Sequences flagged as poor quality0
Sequence length27
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTGTCTGTGTCCTGTCTCTTATACAC3370.9555675277171293No Hit
ACTGTCTCTGTCTCTTATACACATCTC2960.8393115376981314No Hit
ACTGTCTGTGTCCTGTCTCTTCTACAC1230.3487679700569938No Hit
ACTGTCTCTGTCTCTTATACCCATCTC360.10207843026058354No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTCTG353.8127357E-521.4179972
CTGTCTG31300.021.4179972
ACGGTCT800.021.4179971
ACTGCCT303.350667E-421.4179951
ACCGTCT454.9833034E-721.4179951
CCGTCTG454.9833034E-721.4179952
ACTGTCT32100.021.1844671
GTGTCTC1050.020.9319178
AGGTTTT1050.020.93191718
GTCGGGG1050.020.93191710
AGGGGTT250.003379107720.93191516
TGGTTTT651.1823431E-1020.93191518
ACTGTGT405.3169497E-620.9319155
GTGTCTA1300.020.9319158
GTGTTTT1000.020.93191518
GGGTTTT1750.020.93191518
CAGTTTT405.3169497E-620.93191518
GGCATTT303.9152557E-420.93191517
GTGTCGT3150.020.9319158
GTGTCGG5150.020.9319158
Produced by FastQC (version 0.11.2)