FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate6_24.3510000005f018.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate6_24.3510000005f018.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22633
Sequences flagged as poor quality0
Sequence length27
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGTGTGGCTGTCTCTTATACACA2240.9897052975743383No Hit
GTGTAGTGTGGAGGCTGTCTCTTATAC2020.8925020987054302No Hit
GTGTAGTGTGGAGTCTGTCTCTTATAC780.34462952326249285No Hit
GTGTAGTGTGGAGCCTGTCTCTTATAC670.29602792382803866No Hit
GTGTAGTGTGGAAGCTGTCTCTTATAC630.27835461494278263No Hit
GTGTAGTGTGGACCTGTCTCTTATACA590.2606813060575266No Hit
GTGTAGTGTGGAGCTGTCTCTTATACA480.21207970662307252No Hit
GTGTAGTGTGGATGCTGTCTCTTATAC450.1988247249591305No Hit
GTGTAGTGTGGAGACTGTCTCTTATAC450.1988247249591305No Hit
GTGTAGTGTGGAATCTGTCTCTTATAC400.17673308885256042No Hit
GTGTAGTGTGGAGGGGCTGTCTCTTAT310.13696814386073433No Hit
GTGTAGTGTGGACGCTGTCTCTTATAC260.11487650775416428No Hit
GTGTAGTGTGGAACCTGTCTCTTATAC250.11045818053285025No Hit
GTGTAGTGTGGATACTGTCTCTTATAC250.11045818053285025No Hit
GTGTAGTGTGGACCCTGTCTCTTATAC240.10603985331153626No Hit
GTGTAGTGTGGATCCTGTCTCTTATAC230.10162152609022224No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTCGT1650.021.3702031
GTGTAGT19350.021.3702031
GTTTAGT250.002961638821.3702031
AGTAGTG250.00300085621.3220732
GTCGTGT1650.021.3220733
TTTAGTG250.00300085621.3220732
TTAGTGT250.00300085621.3220733
TGTCGTG1650.021.3220732
TGTAGTG19400.021.3220712
GTAGTGT20000.021.2687663
GATGGGG354.4411525E-520.9446911
GAGGTGG354.4411525E-520.9446911
ATGGGGG250.003330222320.9446912
GGAGAGG303.8394073E-420.9446910
GTGTTTT609.458745E-1020.9446918
GGGTTTT1150.020.9446918
GAGCGGG250.003330222320.9446911
CAGTTTT303.8394073E-420.9446918
TAGTGTG20650.020.944694
TATACAC250.003330222320.9446920
Produced by FastQC (version 0.11.2)