FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate6_23.3510000005eff0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate6_23.3510000005eff0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33426
Sequences flagged as poor quality0
Sequence length27
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGCTATCCCTGTCTCTTATACACA1230.3679770238736313No Hit
ATCTGCTATCCTGTCTCTTATACACAT380.1136839585951056No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGCTA404.349402E-621.4134772
AACTGCT404.349402E-621.4134771
TATGCTA250.002956823221.4134772
ATCTACT607.530616E-1021.4134771
TCTACTA607.530616E-1021.4134772
TCTGCTA29850.021.4134772
GCTGCTA709.094947E-1221.4134772
ATGCTAT250.002956823221.4134773
ATATGCT250.002956823221.4134771
ATTTGCT505.7063517E-821.4134771
AGCTGCT709.094947E-1221.4134771
TTTGCTA505.7063517E-821.4134772
CTACTAT607.530616E-1021.4134773
CTGCTAT31150.021.4134753
ATCTGCT29950.021.3419781
TCCTTTT303.9083796E-420.93263618
CCGTGTG303.9083796E-420.93263610
ATCTTTT405.3014883E-620.93263618
CCGTGGT303.9083796E-420.93263610
AATTTTT1600.020.93263619
Produced by FastQC (version 0.11.2)