FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate6_22.3510000005efe3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate6_22.3510000005efe3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37899
Sequences flagged as poor quality0
Sequence length27
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCAGAACTACCCCTGTCTCTTATACA1310.3456555581941476No Hit
GTCAGAACTACCGCTGTCTCTTATACA1050.2770521649647748No Hit
GTCAGAACTACCGGGGGGGGTTTTTTT390.1029050898440592No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGAAC608.6947693E-1021.1922572
TAGAACT608.6947693E-1021.1922573
ACAGAAC250.003149008621.1922572
GACAGAA250.003149008621.1922571
GTCCGAA1750.021.1922571
TAAGAAC250.003149008621.1922572
TCAGAAC33400.021.1922572
GTAAGAA250.003149008621.1922571
AAGAACT250.003149008621.1922573
GTTAGAA608.6947693E-1021.1922571
CAGAACT34500.021.1922573
TCCGAAC1750.021.1922572
GTCAGAA33300.021.1922571
CCGAACT1750.021.1922573
CCAGAAC404.7902668E-621.1922552
GCCAGAA404.7902668E-621.1922551
GCAGAAC455.544971E-721.1922552
GGCAGAA455.544971E-721.1922551
GTGTTTT1350.020.96829818
TACCGGA1450.020.9682989
Produced by FastQC (version 0.11.2)