FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate6_2.3510000005ed6e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate6_2.3510000005ed6e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48625
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTAGTGAGCTAGGGCTGTCTCTTATA530.10899742930591258No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTGAG1250.021.2599163
GTCGTGA2700.021.2599162
TCGTGAG2700.021.2599163
GGGAGTG1250.021.2599161
GCAGTGA900.021.2599162
GGTAGTG41300.021.2599161
GGCAGTG900.021.2599161
CAGTGAG900.021.2599163
GTAGTGA41350.021.2599162
GGAGTGA1250.021.2599162
GGTCGTG2700.021.2599161
GTTAGTG303.5506627E-421.2599141
GTAATGA303.5506627E-421.2599142
TTAGTGA303.5506627E-421.2599142
GGTAATG303.5506627E-421.2599141
TAGTGAG41700.021.2344253
ACTTTTT1400.020.97528519
GATCTGT405.2903524E-620.97528519
TGTTTTT3900.020.97528319
CGTTTTT2950.020.97528319
Produced by FastQC (version 0.11.2)