FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate6_16.3510000005ef29.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate6_16.3510000005ef29.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31979
Sequences flagged as poor quality0
Sequence length27
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAACACCATAGCCTGTCTCTTATACAC2620.8192876575252509No Hit
GAACACCATAGCGGCTGTCTCTTATAC1510.472184871321805No Hit
GAACACCATAGCGCCTGTCTCTTATAC850.2657994308765127No Hit
GAACACCATAGCCCCTGTCTCTTATAC750.234528909596923No Hit
GAACACCATAGCCTGTCTCTTCTACAC740.23140185746896402No Hit
GAACACCATAGCAGCTGTCTCTTATAC640.20013133618937426No Hit
GAACACCATAGCACCTGTCTCTTATAC610.19075017980549736No Hit
GAACACCATAGCTGTCTCTTATACACA590.18449607554957942No Hit
GAACACCATAGCGTCTGTCTCTTATAC520.16260671065386662No Hit
GAACACCATAGCTGCTGTCTCTTATAC480.1500985021420307No Hit
GAACACCATAGCTCCTGTCTCTTATAC450.14071734575815378No Hit
GAACACCATAGCATCTGTCTCTTATAC400.12508208511835892No Hit
GAACACCATAGCGACTGTCTCTTATAC400.12508208511835892No Hit
GAACACCATAGCCGCTGTCTCTTATAC370.11570092873448201No Hit
GAACACCATAGCGCTGTCTCTTATACA330.1031927202226461No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCACCA1000.021.286172
ACACCAT28450.021.286173
AACCCCA1350.021.286172
CCACCAT1000.021.286173
GAACCCC1350.021.286171
GATCACC303.4840227E-421.286171
ATCACCA303.4840227E-421.286172
TCACCAT303.4840227E-421.286173
GACCACC1000.021.286171
GTACACC455.2579344E-721.2861671
TACACCA455.2579344E-721.2861672
GAACACC28000.021.2861671
AACACCA28000.021.2861672
TGGTTTT900.020.95305318
TAGCGGT1200.020.9530539
TAGCGGG2050.020.9530539
TAGCGGA1150.020.9530539
TAGCGCT456.1257924E-720.9530539
TAGCGCG609.858923E-1020.9530539
TAGCGCC405.245034E-620.9530539
Produced by FastQC (version 0.11.2)