FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate6_13.3510000005eeca.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate6_13.3510000005eeca.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12632
Sequences flagged as poor quality0
Sequence length27
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTCTATGGTGGCTGTCTCTTATACA710.5620645978467385No Hit
GACTCTATGGTCTGTCTCTTATACACA690.5462317922735909No Hit
GACTCTATGGTGCCTGTCTCTTATACA450.35623812539582017No Hit
GACTCTATGGTGTCTGTCTCTTATACA400.31665611146295125No Hit
GACTCTATGGTGACTGTCTCTTATACA230.18207726409119696No Hit
GACTCTATGGTGCTGTCTCTTATACAC130.10291323622545914No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATATG607.057679E-1021.216873
ACTCTAT11250.021.2168672
CTCTATG11400.021.2168673
GACTCTA11250.021.2168671
TTGTTTT303.6863604E-420.96428718
TGGTTTT506.300252E-820.96428718
GTGTTTT303.6863604E-420.96428718
GGTGAGG455.509337E-720.9642879
GGTGAGA250.003231946420.9642879
GCCTTTT250.003231946420.96428718
GGTGAAT250.003231946420.9642879
AAATTTT303.6863604E-420.96428718
TATATGG608.221832E-1020.9642874
AATTTTT557.19956E-920.96428719
CCTGTTT250.003231946420.96428717
GGATTTT455.509337E-720.96428718
CATTTTT557.19956E-920.96428719
ACTTTTT250.003231946420.96428719
GGCCTGT250.003231946420.96428718
TGCTTTT250.003231946420.96428718
Produced by FastQC (version 0.11.2)