FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate6_12.3510000005ee94.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate6_12.3510000005ee94.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25856
Sequences flagged as poor quality0
Sequence length27
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCCTTACGCCTGTCTCTTATACAC1230.47571163366336633No Hit
GAATCCTTACGCCTGTCTCTTCTACAC390.15083539603960397No Hit
GAATCCTTACGCTGTCTCTTATACACA280.1082920792079208No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCCTT22500.021.2829072
CTCCTTA800.021.2829073
ACTCCTT800.021.2829072
GTATCCT455.183938E-721.2829071
TTCCTTA455.183938E-721.2829073
TATCCTT455.183938E-721.2829072
GAATCCT22500.021.2829071
GATTCCT455.183938E-721.2829071
AATACTT1050.021.2829072
GAATACT1050.021.2829071
ATACTTA1050.021.2829073
ATTCCTT455.183938E-721.2829072
GACTCCT800.021.2829071
ATCCTTA22850.021.2829073
TACGCTG2550.020.953588
ACGCGGG2150.020.953589
AGCCTGT250.003334921320.95357918
TTGTTTT651.1277734E-1020.95357918
TGGTTTT609.604264E-1020.95357918
GCGGGGC250.003334921320.95357911
Produced by FastQC (version 0.11.2)