FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate6_11.3510000005ee87.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate6_11.3510000005ee87.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40793
Sequences flagged as poor quality0
Sequence length27
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGTGCCTCAGCTGTCTCTTATACACA470.11521584585590665No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGTGCC658.54925E-1121.4760721
GTCCCTC303.3022085E-421.476073
ATTGCCT505.610127E-821.476072
AAGTGCC37650.021.476071
AGTCCCT303.3022085E-421.476072
TTGCCTC505.610127E-821.476073
AATTGCC505.610127E-821.476071
AAGTCCC303.3022085E-421.476071
GTGCCTC38250.021.476073
AAGTACC556.4428605E-921.4760681
GTACCTC556.4428605E-921.4760683
AGTACCT556.4428605E-921.4760682
AGTGCCT37800.021.3624422
TCCTTTT303.941528E-420.922718
CAGTCGT601.029548E-920.92279
CAGTCGG1050.020.92279
CAGTCGC601.029548E-920.92279
CAGTTGT601.029548E-920.92279
GCCTCAG39850.020.92275
CAGTCCC303.941528E-420.92279
Produced by FastQC (version 0.11.2)