FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate6_10.3510000005ee6d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate6_10.3510000005ee6d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23227
Sequences flagged as poor quality0
Sequence length27
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGTCTCTGCATCTGTCTCTTATACAC2431.0461962371378137No Hit
ACGTCTCTGCCTGTCTCTTATACACAT1030.44344943384853835No Hit
ACGTCTCTGCATCTGTCTCTTCTACAC700.3013734016446377No Hit
ACGTCTCTGCCTGTCTCTTATCCACAT340.14638136651310973No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTC19850.021.2757131
CGTCTCT19850.021.2757132
GTATCTG1700.021.2757113
ACGTATC1700.021.2757111
GTCTCTG20600.021.2757113
CGTATCT1700.021.2757112
ATGTCTC751.8189894E-1221.2757111
ACTTCTC250.003041712321.275711
TTCTCTG250.003041712321.275713
CTTCTCT250.003041712321.275712
TGGTTTT651.09139364E-1020.95474218
TGCATTC1100.020.9547428
TGCATTA1250.020.9547428
GCCTTTT303.831254E-420.95474218
CAGTTTT455.9721606E-720.95474218
TGCATCG1650.020.9547428
CATGACG303.831254E-420.95474210
TTACTTT303.831254E-420.95474217
GATATTT303.831254E-420.95474217
AATTTTT900.020.95474219
Produced by FastQC (version 0.11.2)