FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate6_1.3510000005ed38.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate6_1.3510000005ed38.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34740
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTCTGCTCCTGTCTCTTATACACAT900.2590673575129534No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTTTG404.577989E-621.2928261
GTGTATG2200.021.2928261
GCGTCTG250.0030582221.2928241
GTCTGCT30950.021.2616983
GTATGCT2200.021.2616963
GTTTGCT404.6367404E-621.2616963
GGTCTGC354.0332125E-521.2616962
TGTTTGC404.6367404E-621.2616962
AGTCTGC404.6367404E-621.2616962
TGTATGC2200.021.2616962
CGTCTGC250.003084378521.2616962
TGTCTGC30100.021.2616942
GTGTCTG30200.021.2223191
CTCATGT2350.020.9553348
TCCTTTT303.88411E-420.95533218
GCCTTTT303.88411E-420.95533218
CATTGGG354.5131994E-520.95533210
ACGTTTT354.5131994E-520.95533218
TTTCTGT558.456482E-920.95533221
TATGCTC2200.020.9553324
Produced by FastQC (version 0.11.2)