FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate5_96.3510000005ed1e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate5_96.3510000005ed1e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35200
Sequences flagged as poor quality0
Sequence length27
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAGCGTGGAACCTGTCTCTTATACAC4321.2272727272727273No Hit
TAAGCGTGGAACCTGTCTCTTCTACAC1410.40056818181818177No Hit
TAAGCGTGGAACTGTCTCTTATACACA490.13920454545454547No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGTGG607.912604E-1021.338643
TAAGCGT30550.021.338641
AAGAGTG1350.021.338642
ATGCGTG607.912604E-1021.338642
TAAGAGT1350.021.338641
TACGCGT1400.021.338641
AGAGTGG1350.021.338643
AAGCGTG30550.021.338642
TTAGCGT455.165457E-721.338641
AGCGTGG31050.021.3042773
TGGTTTT1350.020.94460318
AACGTTA354.533449E-520.94460310
AACGCGT354.533449E-520.94460310
AAATTTT701.4551915E-1120.94460318
AACGATG354.533449E-520.94460310
AATTTTT2550.020.94460319
GAGTGGA1350.020.9446034
AGGATTT354.533449E-520.94460317
AACGAGA354.533449E-520.94460310
TCGGTTT354.533449E-520.94460317
Produced by FastQC (version 0.11.2)