FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate5_88.3510000005ec2c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate5_88.3510000005ec2c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39677
Sequences flagged as poor quality0
Sequence length27
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGAGCTTGACCTGTCTCTTATACAC2600.6552914786904251No Hit
GTTGAGCTTGACCTGTCTCTTCTACAC1050.2646369433172871No Hit
GTTGAGCTTGACGCTGTCTCTTATACA600.1512211104670212No Hit
GTTGAGCTTGACCCTGTCTCTTATACA550.1386193512614361No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAGC404.4188655E-621.3943311
GGTGAGC556.719347E-921.3943311
GTGAGCT556.719347E-921.3943312
AGAGCTT404.4188655E-621.3943313
TAGAGCT404.4188655E-621.3943312
GTTGAGC32900.021.394331
CTGAGCT250.002982256521.394332
TTGAGCT32900.021.394332
TGGAGCT900.021.394332
GATGAGC250.002982256521.394331
GCTGAGC250.002982256521.394331
GTGGAGC900.021.394331
TGAGCTT33950.021.394333
GGAGCTT900.021.394333
ATGAGCT250.002982256521.394332
GTTGCGC2850.021.0189911
AGGGGTT250.003382957820.93568816
TGGTTTT1600.020.93568818
ACTGTGC250.003382957820.93568811
CAGTTTT1000.020.93568818
Produced by FastQC (version 0.11.2)