FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate5_80.3510000005eb10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate5_80.3510000005eb10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49479
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATAGTTAGCCCTGTCTCTTATACAC2540.5133490975969603No Hit
GGATAGTTAGCCTGTCTCTTATACACA1280.25869560823783827No Hit
GGATAGTTAGCCCCTGTCTCTTATACA630.12732674467956104No Hit
GGATAGTTAGCCGCTGTCTCTTATACA600.12126356636148669No Hit
GGATAGTTAGCTGTCTCTTATACACAT550.11115826916469614No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTAGTT2250.021.4859812
GTATAGT250.00291932521.4859811
GGATAGT41150.021.4859811
ATAGTTA41400.021.4859813
CTAGTTA2250.021.4859813
GGCTAGT2250.021.4859811
TTAGTTA1600.021.4859813
TATAGTT250.00291932521.4859812
GATAGTT41150.021.4859812
GATCGTT2850.021.1090342
GGATCGT2850.021.1090341
ATCGTTA2850.021.1090343
AGCCGTG1150.020.9211359
AGCCGCT1150.020.9211359
CCGCGAG303.9600572E-420.92113311
AGGGGTT250.003408410420.92113316
GCCCTAG354.6214253E-520.92113310
ACTGTTT354.6214253E-520.92113317
AGCCCGG1600.020.9211339
AGCCCGA800.020.9211339
Produced by FastQC (version 0.11.2)