FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate5_8.3510000005e219.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate5_8.3510000005e219.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67570
Sequences flagged as poor quality0
Sequence length27
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAGTGCTCATGCTGTCTCTTATACAC14812.1918010951605744No Hit
ACAGTGCTCATGGGCTGTCTCTTATAC2560.3788663608110108No Hit
ACAGTGCTCATGGCTGTCTCTTATACA1330.19683291401509545No Hit
ACAGTGCTCATGGCCTGTCTCTTATAC1120.16575403285481724No Hit
ACAGTGCTCCTGTCTCTTATACACATC1090.1613141926890632No Hit
ACAGTGCTCATGAGCTGTCTCTTATAC890.131715258250703No Hit
ACAGTGCTCATGGTCTGTCTCTTATAC800.11839573775344087No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGCGC404.5195193E-621.3824631
ACAGTGC58500.021.3824631
ACTGTGC950.021.3824631
ACCGTGC4300.021.3824631
ATAGTGC701.0913936E-1121.3824631
CCGTGCT4300.021.3663142
TAGTGCT701.0913936E-1121.3663142
AGAGCTC800.021.3663143
AGTGCTC59350.021.3663143
CTGTGCT950.021.3663142
CGTGCTC4300.021.3663143
CAGCGCT404.549296E-621.3663142
CAGTGCT58550.021.3663142
ACTTTTT2300.020.93930619
TCATGAG4600.020.9393068
CTCATGG28250.020.9393067
GACTTTT1150.020.93930618
CATGCGC1150.020.9393069
ATGGGTT558.902134E-920.93930410
ACTGTTT303.957621E-420.93930417
Produced by FastQC (version 0.11.2)