FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate5_79.3510000005eaf5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate5_79.3510000005eaf5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47246
Sequences flagged as poor quality0
Sequence length27
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGACATCGCAACCTGTCTCTTATACA540.1142953900859332No Hit
CAGACATCGCAAGCTGTCTCTTATACA510.10794564619227025No Hit
CAGACATCGCAAAAAAAAAAAAAAAAA490.10371248359649493No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGAAAT1950.021.1754021
CATACAT608.949428E-1021.1754021
CAGCCAT1100.021.17541
CTGACAT651.05501385E-1021.17541
GAAATCG1950.021.1527793
GACATCG42700.021.1527793
AGAAATC1950.021.1527792
AGACATC42150.021.1527792
AGCCATC1100.021.1527772
TGACATC651.05501385E-1021.1527772
TACATCG609.076757E-1021.1527773
GCCATCG1100.021.1527773
ATACATC609.076757E-1021.1527772
CAGACAT42200.021.1252231
AAATCGC1950.020.9735184
CATTTTT2100.020.97351819
ACATCGC43750.020.9735184
TGCTTTT1050.020.97351818
GCGTTTT1050.020.97351818
TCGCAAT11800.020.9735187
Produced by FastQC (version 0.11.2)