FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate5_72.3510000005ea11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate5_72.3510000005ea11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30266
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGTGTGGCTGTCTCTTATACACA3701.2224938875305624No Hit
GTGTAGTGTGGAGGCTGTCTCTTATAC3301.0903323861759069No Hit
GTGTAGTGTGGAGTCTGTCTCTTATAC1560.515429855283156No Hit
GTGTAGTGTGGATGCTGTCTCTTATAC1440.4757814048767594No Hit
GTGTAGTGTGGAGCCTGTCTCTTATAC1410.4658692922751602No Hit
GTGTAGTGTGGAAGCTGTCTCTTATAC1180.38987642899623337No Hit
GTGTAGTGTGGAGCTGTCTCTTATACA1160.3832683539285006No Hit
GTGTAGTGTGGAGACTGTCTCTTATAC1120.3700522037930351No Hit
GTGTAGTGTGGACCTGTCTCTTATACA1030.3403158659882376No Hit
GTGTAGTGTGGACGCTGTCTCTTATAC820.27093107777704356No Hit
GTGTAGTGTGGAATCTGTCTCTTATAC800.26432300270931075No Hit
GTGTAGTGTGGATACTGTCTCTTATAC650.214762439701315No Hit
GTGTAGTGTGGATCTGTCTCTTATACA590.19493821449811669No Hit
GTGTAGTGTGGATCCTGTCTCTTATAC550.18172206436265115No Hit
GTGTAGTGTGGACCCTGTCTCTTATAC520.17180995176105202No Hit
GTGTAGTGTGGAACCTGTCTCTTATAC510.1685059142271856No Hit
GTGTAGTGTGGATTCTGTCTCTTATAC490.16189783915945286No Hit
GTGTAGTGTGGAAACTGTCTCTTATAC470.15528976409172007No Hit
GTGTAGTGTGGAGGGGCTGTCTCTTAT330.10903323861759069No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTCGT1850.021.364251
GTCGTGT1850.021.364253
GTGTAGT26250.021.364251
GGGTAGT455.055499E-721.364251
TGTAGTG26250.021.364252
GGTAGTG455.055499E-721.364252
TGTCGTG1850.021.364252
GTAGTGT27300.021.364253
CGTAGTG353.8637285E-521.3642482
TGGTTTT800.020.94049618
GTGTTTT900.020.94049618
CAGTTTT456.138689E-720.94049618
TAGTGTG28050.020.9404964
TTTCTGT405.2569158E-620.94049621
AGGGTTT456.138689E-720.94049617
CATTTTT1050.020.94049619
TGGAGTA651.1641532E-1020.9404969
TGGAGGT1300.020.9404969
TGGAGGA1050.020.9404969
TGGAGCT651.1641532E-1020.9404969
Produced by FastQC (version 0.11.2)