FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate5_71.3510000005e9f6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate5_71.3510000005e9f6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57573
Sequences flagged as poor quality0
Sequence length27
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGCTATCCCTGTCTCTTATACACA3900.6774008649887969No Hit
ATCTGCTATCCTGTCTCTTATACACAT1450.25185416775224495No Hit
ATCTGCTATCCGGCTGTCTCTTATACA730.12679554652354402No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTGCT52400.021.44881
ACTGCTA404.380174E-621.4487972
TATGCTA505.793663E-821.4487972
ATCTACT751.8189894E-1221.4487971
TCTACTA751.8189894E-1221.4487972
TCTGCTA52450.021.4487972
GCTGCTA556.686605E-921.4487972
ATGCTAT505.793663E-821.4487973
ATATGCT505.793663E-821.4487971
ATTTGCT455.0309063E-721.4487971
CTGCTAT53700.021.4487973
TTTGCTA455.0309063E-721.4487972
CTACTAT751.8189894E-1221.4487973
TTGCTAT455.0309063E-721.4487973
ATCTCCT607.730705E-1021.4487951
CCTGCTA303.3546455E-421.4487952
ACCTGCT303.3546455E-421.4487951
TCTCCTA607.730705E-1021.4487952
ACTGTTT405.4258653E-620.92702117
TGCATTT354.628382E-520.92702117
Produced by FastQC (version 0.11.2)