FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate5_7.3510000005e1fe.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate5_7.3510000005e1fe.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences151856
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCAGCAATGCCTGTCTCTTATACAC6290.41420819723949004No Hit
CTTCAGCAATGCGGGGGGGGTTTTTTT1880.1238014961542514No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACAGC1500.021.2693841
CTTCAGC126450.021.2693841
CTTAAGC800.021.2693841
ATCAGCA557.64885E-921.262282
TTCAGCA126700.021.262282
GTCAGCA1000.021.262282
ACAGCAA1500.021.2622783
TACAGCA1500.021.2622782
CCAGCAA1800.021.2622783
TAAGCAA800.021.2622783
TCGGCAA751.8189894E-1221.2622783
TCCAGCA1800.021.2622782
TCTGCAA1200.021.2622783
TTAAGCA800.021.2622782
TCAGCAA129000.021.2540383
CTTCCGC9550.021.1580281
TTCCGCA9550.021.1509572
TCCGCAA9550.021.1509573
CTGCAGC4850.021.0501141
GTAGATT250.00340282220.96113416
Produced by FastQC (version 0.11.2)