FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate5_64.3510000005e912.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate5_64.3510000005e912.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58953
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAACACCATAGCCTGTCTCTTATACAC5870.9957084457109902No Hit
GAACACCATAGCGGCTGTCTCTTATAC2710.45968822621410277No Hit
GAACACCATAGCGCCTGTCTCTTATAC1850.31380930571811444No Hit
GAACACCATAGCGTCTGTCTCTTATAC1510.2561362441266772No Hit
GAACACCATAGCTGTCTCTTATACACA1490.25274371109188676No Hit
GAACACCATAGCAGCTGTCTCTTATAC1440.2442623785049107No Hit
GAACACCATAGCACCTGTCTCTTATAC1170.19846318253523995No Hit
GAACACCATAGCATCTGTCTCTTATAC1080.18319678387868302No Hit
GAACACCATAGCCCCTGTCTCTTATAC1050.17810798432649738No Hit
GAACACCATAGCCGCTGTCTCTTATAC1000.16962665173952132No Hit
GAACACCATAGCGACTGTCTCTTATAC1000.16962665173952132No Hit
GAACACCATAGCCCTGTCTCTTATACA950.16114531915254526No Hit
GAACACCATAGCTGCTGTCTCTTATAC900.15266398656556918No Hit
GAACACCATAGCTTCTGTCTCTTATAC760.1289162553220362No Hit
GAACACCATAGCTCCTGTCTCTTATAC740.12552372228724576No Hit
GAACACCATAGCAACTGTCTCTTATAC700.11873865621766491No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACCAT250.003044012721.3405363
ACCACCA2800.021.3405362
GAAAACC250.003044012721.3405361
ACACCAT51500.021.3405363
GTACACC659.640644E-1121.3405361
AACCCCA2250.021.3405362
TACACCA659.640644E-1121.3405362
CCACCAT2800.021.3405363
GAACCCC2250.021.3405361
ACCCCAT2250.021.3405363
GATCACC751.8189894E-1221.3405361
ATCACCA751.8189894E-1221.3405362
AAAACCA250.003044012721.3405362
TCACCAT751.8189894E-1221.3405363
GACCACC2800.021.3405361
GAACACC50750.021.3405341
AACACCA50750.021.3405342
TTTTCTG354.5747365E-520.96010221
GTTCTGT303.922919E-420.960120
TTTTTCT303.922919E-420.960121
Produced by FastQC (version 0.11.2)