FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n01_kb_plate5_56.3510000005e820.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n01_kb_plate5_56.3510000005e820.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43164
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAGTGCTCATGCTGTCTCTTATACAC9812.272727272727273No Hit
ACAGTGCTCATGCTGTCTCTTCTACAC3080.7135575942915392No Hit
ACAGTGCTCATGGGCTGTCTCTTATAC2010.4656658326383097No Hit
ACAGTGCTCATGGCTGTCTCTTATACA1130.2617922342692985No Hit
ACAGTGCTCATGGCCTGTCTCTTATAC1100.254841997961264No Hit
ACAGTGCTCATGGTCTGTCTCTTATAC950.22009081642109166No Hit
ACAGTGCTCATGAGCTGTCTCTTATAC640.14827170790473543No Hit
ACAGTGCTCCTGTCTCTTATACACATC550.127420998980632No Hit
ACAGTGCTCATGCCTGTCTCTTATACA520.12047076267259753No Hit
ACAGTGCTCATGTGCTGTCTCTTATAC510.11815401723658606No Hit
ACAGTGCTCATGCGCTGTCTCTTATAC500.11583727180057456No Hit
ACAGTGCTCATGACCTGTCTCTTATAC460.1065702900565286No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGCGC250.002995461521.3834321
ACAGTGC37700.021.3834321
ACTGTGC556.8030204E-921.3834321
ACAGAGC404.454003E-621.3834321
ACCGTGC2750.021.3834321
CAGAGCT404.454003E-621.3834322
AGAGCTC404.454003E-621.3834323
AGTGCTC38250.021.3834323
ATAGTGC556.8030204E-921.3834321
CTGTGCT556.8030204E-921.3834322
CGTGCTC2750.021.3834323
AGCGCTC250.002995461521.3834323
CAGCGCT250.002995461521.3834322
CAGTGCT37750.021.355112
CCGTGCT2800.021.0015852
TCATGCC1850.020.937438
TCATGAA1350.020.937438
CATGGGG2700.020.937439
ATGGGTC303.928041E-420.93742810
TCCTTTT507.376366E-820.93742818
Produced by FastQC (version 0.11.2)